An alternative workflow for molecular detection of SARS-CoV-2 - escape from the NA extraction kit-shortage, Copenhagen, Denmark, March 2020.
Identifieur interne : 000A03 ( Main/Exploration ); précédent : 000A02; suivant : 000A04An alternative workflow for molecular detection of SARS-CoV-2 - escape from the NA extraction kit-shortage, Copenhagen, Denmark, March 2020.
Auteurs : Anna S. Fomsgaard [Danemark] ; Maiken Wors E Rosenstierne [Danemark]Source :
- Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin [ 1560-7917 ] ; 2020.
Descripteurs français
- KwdFr :
- ARN viral (analyse), ARN viral (génétique), ARN viral (isolement et purification), Coronavirus (génétique), Coronavirus (isolement et purification), Danemark, Flux de travaux, Humains, Infections à coronavirus (diagnostic), Pandémies, Partie orale du pharynx (virologie), Pneumopathie virale (diagnostic), RT-PCR (), Sensibilité et spécificité, Syndrome respiratoire aigu sévère (diagnostic).
- MESH :
- analyse : ARN viral.
- diagnostic : Infections à coronavirus, Pneumopathie virale, Syndrome respiratoire aigu sévère.
- génétique : ARN viral, Coronavirus.
- isolement et purification : ARN viral, Coronavirus.
- virologie : Partie orale du pharynx.
- Danemark, Flux de travaux, Humains, Pandémies, RT-PCR, Sensibilité et spécificité.
- Wicri :
- geographic : Danemark.
English descriptors
- KwdEn :
- Betacoronavirus, Coronavirus (genetics), Coronavirus (isolation & purification), Coronavirus Infections (diagnosis), Denmark, Humans, Oropharynx (virology), Pandemics, Pneumonia, Viral (diagnosis), RNA, Viral (analysis), RNA, Viral (genetics), RNA, Viral (isolation & purification), Reverse Transcriptase Polymerase Chain Reaction (methods), Sensitivity and Specificity, Severe Acute Respiratory Syndrome (diagnosis), Workflow.
- MESH :
- chemical , analysis : RNA, Viral.
- chemical , genetics : RNA, Viral.
- chemical , isolation & purification : RNA, Viral.
- geographic : Denmark.
- diagnosis : Coronavirus Infections, Pneumonia, Viral, Severe Acute Respiratory Syndrome.
- genetics : Coronavirus.
- isolation & purification : Coronavirus.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- virology : Oropharynx.
- Betacoronavirus, Humans, Pandemics, Sensitivity and Specificity, Workflow.
Abstract
The World Health Organization has declared COVID-19 caused by the newly discovered SARS-CoV-2 a pandemic. Due to growing demand for reagents and/or kits to extract SARS-CoV-2 RNA for subsequent RT-qPCR diagnostics, there is a worldwide risk of shortages. With a detection sensitivity of 97.4% (95% CI: 86.2-99.9%), we describe a simple, fast, alternative workflow for molecular detection of SARS-CoV-2, where samples are simply heat-processed for 5 min at 98 °C before a commonly-used RT-qPCR procedure.
DOI: 10.2807/1560-7917.ES.2020.25.14.2000398
PubMed: 32290902
Affiliations:
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Le document en format XML
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xml:lang="en">The World Health Organization has declared COVID-19 caused by the newly discovered SARS-CoV-2 a pandemic. Due to growing demand for reagents and/or kits to extract SARS-CoV-2 RNA for subsequent RT-qPCR diagnostics, there is a worldwide risk of shortages. With a detection sensitivity of 97.4% (95% CI: 86.2-99.9%), we describe a simple, fast, alternative workflow for molecular detection of SARS-CoV-2, where samples are simply heat-processed for 5 min at 98 °C before a commonly-used RT-qPCR procedure.</div></front></TEI><affiliations><list><country><li>Danemark</li></country><region><li>Hovedstaden</li></region><settlement><li>Copenhague</li></settlement></list><tree><country name="Danemark"><region name="Hovedstaden"><name sortKey="Fomsgaard, Anna S" sort="Fomsgaard, Anna S" uniqKey="Fomsgaard A" first="Anna S" last="Fomsgaard">Anna S. Fomsgaard</name></region><name sortKey="Rosenstierne, Maiken Wors E" sort="Rosenstierne, Maiken Wors E" uniqKey="Rosenstierne M" first="Maiken Wors E" last="Rosenstierne">Maiken Wors E Rosenstierne</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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